The VIPcheck™ method consists of a 4-wells plate, which contains agar supplemented with 3 medical azoles (Voriconazole, Itraconazole and Posaconazole, VIP) and a growth control. The assay is rapid, requires no specific technical skills, and detects all azole resistance mechanisms, and therefore can be used in every microbiology laboratory. VIPcheck™ results can be reliably read after one day if growth of Aspergillus fumigatus is visible, and in all isolates after 2 days of incubation at 35-37°C.
Multiple Aspergillus fumigatus colonies from a patients culture can be tested using a single VIPcheck™-plate, and if a mixed azole-susceptible and azole-resistant colonies are present, the azole-resistant colonies will grow. We recommend to determine the in vitro susceptibility (MIC) for all colonies that grow on any of the agars that contain an azole.