Clostridium botulinum is a rod-shaped, anaerobic, spore-forming bacterium capable of inducing foodborne botulism when consumed in contaminated food. From the eight different toxin types A to H, only A, B, E and F are known to cause disease in humans. Improperly preserved foods, such as canned goods or pickled fish, are the most common form of contracting foodborne botulism. C. botulinum is a naturally occurring soil bacterium. Thus, it can potentially contaminate food at multiple stages within the production process, including honey and milk.

The foodproof® Clostridium botulinum Detection LyoKit is based on real-time PCR technology, which is well-established in the food industry as a highly sensitive and specific detection method. The kit detects and identifies all of the human diseasecausing toxin types (A, B, E and F) in one multiplex PCR assay using melting curve analysis. This method is a superior alternative to other assays, including mouse bioassay. 

Real-time PCR method, based on ISO/TS 17919. 

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